【求助】blast结果分析

我参考外文文献的做Cx43基因PCR 引物,用pubmed的blast结果,因为是新手,也看了园子里一些帖子,还是不怎么会分析,请大家帮我看看这个引物可以用不,十分感谢!
gi|33285446|ref|NM_012567.2| Rattus norvegicus gap junction m... 42.1 0.075
gi|18250447|emb|AL139098.15| Human DNA sequence from clone RP... 42.1 0.075
gi|20072865|gb|BC026329.1| Homo sapiens gap junction protein,... 42.1 0.075
gi|51859469|gb|BC081842.1| Rattus norvegicus gap junction mem... 42.1 0.075
gi|55978|emb|X06656.1|RNCONN43 Rat heart mRNA for gap junction p 42.1 0.075
gi|4755136|ref|NM_000165.2| Homo sapiens gap junction protein... 42.1 0.075
gi|109072851|ref|XM_001109617.1| PREDICTED: Macaca mulatta co... 42.1 0.075
gi|109072849|ref|XM_001109566.1| PREDICTED: Macaca mulatta co... 42.1 0.075
gi|39981118|gb|AY382588.1| Cercopithecus aethiops connexin 43 ge 42.1 0.075
gi|67971111|dbj|AB169817.1| Macaca fascicularis brain cDNA, c... 42.1 0.075
gi|49456280|emb|CR541660.1| Homo sapiens full open reading fr... 42.1 0.075
gi|32816616|gb|AY324140.1| Rattus norvegicus gap junction mem... 42.1 0.075
gi|6563407|gb|AF151980.2|AF151980 Homo sapiens connexin 43 (CX43 42.1 0.075
gi|62898138|dbj|AK223289.1| Homo sapiens mRNA for connexin 43 va 42.1 0.075
gi|60654370|gb|AY892959.1| Synthetic construct Homo sapiens c... 42.1 0.075
gi|61363755|gb|AY890497.1| Synthetic construct Homo sapiens c... 42.1 0.075
gi|60827372|gb|AY893759.1| Synthetic construct Homo sapiens c... 42.1 0.075
gi|29916|emb|X52947.1|HSCGJP Human mRNA for cardiac gap junction 42.1 0.075
gi|181208|gb|M65188.1|HUMCX43 Human connexin 43 (GJA1, Cx43) mRN 42.1 0.075
gi|39981122|gb|AY382590.1| Oryctolagus cuniculus connexin 43 gen 40.1 0.29
gi|38609727|gb|AY462223.1| Canis familiaris connexin 43 (Gja1) m 40.1 0.29
gi|21844555|gb|AC010228.8| Homo sapiens chromosome 5 clone CTC-2 40.1 0.29
gi|34419626|ref|NG_003029.1| Homo sapiens gap junction protei... 40.1 0.29
gi|6102634|gb|AC011421.2|AC011421 Homo sapiens chromosome 5 clon 40.1 0.29
gi|50950160|ref|NM_001002951.1| Canis familiaris gap junction... 40.1 0.29
gi|181210|gb|M65189.1|HUMCX43PS Human connexin 43 processed pseu 40.1 0.29
gi|28975324|gb|AY206456.1| Cricetulus griseus gap junction pr... 38.2 1.2
gi|28975322|gb|AY206455.1| Mesocricetus auratus gap junction ... 38.2 1.2
gi|39981128|gb|AY382593.1| Sus scrofa connexin 43 gene, partial 38.2 1.2
gi|39981126|gb|AY382592.1| Spermophilus citellus connexin 43 gen 38.2 1.2
gi|39981124|gb|AY382591.1| Phodopus sungorus connexin 43 gene, p 38.2 1.2
gi|42544094|gb|AC131105.4| Mus musculus BAC clone RP23-280N9 ... 38.2 1.2
gi|39752885|gb|AC132957.3| Mus musculus BAC clone RP23-149E9 ... 38.2 1.2
gi|55294919|emb|BX547998.9| Zebrafish DNA sequence from clone... 36.2 4.6
gi|22213375|gb|AC104036.8| Homo sapiens chromosome 8, clone RP11 36.2 4.6
gi|71725580|gb|AC155807.3| Mus musculus BAC clone RP24-303N21... 36.2 4.6
gi|21070323|gb|AF508037.1| Mesocricetus auratus connexin 47 mRNA 36.2 4.6
gi|26087706|dbj|AK040228.1| Mus musculus 0 day neonate thymus... 36.2 4.6
gi|19849385|gb|AC015476.10| Homo sapiens, clone RP11-16G11, comp 36.2 4.6
gi|68378929|ref|XM_683374.1| PREDICTED: Danio rerio similar t... 36.2 4.6
gi|68342226|gb|AC152718.6| Mus musculus BAC clone RP24-432B3 ... 36.2 4.6
gi|22779223|dbj|AP005358.3| Homo sapiens genomic DNA, chromos... 36.2 4.6
gi|8072467|gb|AC009567.8|AC009567 Homo sapiens chromosome 4, clo 36.2 4.6
gi|25141111|gb|AC084125.9| Homo sapiens chromosome 8, clone CTD- 36.2 4.6
gi|61403389|gb|BC091904.1| Danio rerio cDNA clone IMAGE:7149102 36.2 4.6
gi|21727418|emb|AL672195.9| Mouse DNA sequence from clone RP2... 36.2 4.6
gi|25896305|emb|AL928738.8| Mouse DNA sequence from clone RP2... 36.2 4.6
gi|76253341|emb|CR974460.5| Pig DNA sequence from clone PigE-... 34.2 18
gi|21687178|ref|NM_010288.2| Mus musculus gap junction membra... 34.2 18
gi|71274021|emb|CR942185.8| Human DNA sequence from clone DAM... 34.2 18
gi|38502258|emb|BX842590.1| Rhesus DNA sequence from clone bRH-1 34.2 18
gi|71059982|emb|CT010327.1| Mus musculus full open reading fr... 34.2 18
gi|56334484|emb|CR666673.2|CNS0FDDJ Tetraodon nigroviridis full- 34.2 18
gi|110759082|ref|XM_396503.3| PREDICTED: Apis mellifera simil... 34.2 18
gi|11228407|emb|AL031682.4|HS873P14 Human DNA sequence from c... 34.2 18
gi|13905195|gb|BC006894.1| Mus musculus gap junction membrane... 34.2 18
gi|38490288|emb|BX842578.1| Mycobacterium tuberculosis H37Rv com 34.2 18
gi|31618479|emb|BX248340.1| Mycobacterium bovis subsp. bovis ... 34.2 18
gi|21665918|emb|AL591129.24| Mouse DNA sequence from clone RP... 34.2 18
gi|109509075|ref|XR_009534.1| PREDICTED: Rattus norvegicus si... 34.2 18
gi|109508284|ref|XM_226572.4| PREDICTED: Rattus norvegicus si... 34.2 18
gi|50952454|gb|AE000516.2| Mycobacterium tuberculosis CDC1551, c 34.2 18
gi|50284585|gb|AC115858.14| Mus musculus chromosome 7, clone RP2 34.2 18
gi|23503497|dbj|AP003816.2| Oryza sativa (japonica cultivar-g... 34.2 18
gi|58531194|dbj|AP008213.1| Oryza sativa (japonica cultivar-g... 34.2 18
gi|30524938|gb|AC122356.4| Mus musculus BAC clone RP23-401P15... 34.2 18
gi|15030142|gb|BC011324.1| Mus musculus gap junction membrane... 34.2 18
gi|24476165|gb|AC121823.3| Mus musculus BAC clone RP23-466I14... 34.2 18
gi|28973963|gb|AC124720.3| Mus musculus BAC clone RP23-387F14... 34.2 18
gi|27657612|gb|AC122873.5| Mus musculus BAC clone RP23-110K10 fr 34.2 18
gi|11597176|gb|AC015983.7| Homo sapiens BAC clone RP11-560I19 fr 34.2 18
gi|33286887|gb|BC055375.1| Mus musculus gap junction membrane... 34.2 18
gi|94382620|ref|XM_001000131.1| PREDICTED: Mus musculus RIKEN... 34.2 18
gi|94381164|ref|XM_983220.1| PREDICTED: Mus musculus RIKEN cD... 34.2 18
gi|94380907|ref|XM_908793.2| PREDICTED: Mus musculus RIKEN cD... 34.2 18
gi|73808249|gb|AC113299.14| Mus musculus chromosome 5, clone RP2 34.2 18
gi|42627474|emb|BX927320.5| Human DNA sequence from clone DAM... 34.2 18
gi|71834840|gb|AC111086.18| Mus musculus chromosome 7, clone RP2 34.2 18
gi|21699195|gb|AC084335.6| Homo sapiens chromosome 18, clone ... 34.2 18
gi|71533313|gb|AC154394.6| Mus musculus chromosome 18, clone RP2 34.2 18
gi|26331851|dbj|AK036979.1| Mus musculus adult female vagina ... 34.2 18
gi|20377017|gb|AC087274.8| Homo sapiens chromosome 8, clone RP11 34.2 18
gi|26081534|dbj|AK029814.1| Mus musculus adult male testis cD... 34.2 18
gi|26325241|dbj|AK029291.1| Mus musculus 0 day neonate head c... 34.2 18
gi|16506920|gb|AC097657.3| Homo sapiens BAC clone RP11-362J18 fr 34.2 18
gi|18464204|gb|AC025480.7| Homo sapiens chromosome 18, clone RP1 34.2 18
gi|74216957|dbj|AK145692.1| Mus musculus blastocyst blastocys... 34.2 18
gi|38257216|gb|AC145335.15| Pan troglodytes clone rp43-26k12, co 34.2 18
gi|26349290|dbj|AK081668.1| Mus musculus 16 days embryo head ... 34.2 18
gi|29126443|gb|AC048387.16| Homo sapiens chromosome 8, clone RP1 34.2 18
gi|68268281|gb|AC125227.6| Mus musculus BAC clone RP23-42I13 ... 34.2 18
gi|63004118|gb|AC159214.2| Pan troglodytes BAC clone CH251-52... 34.2 18
gi|74179078|dbj|AK171955.1| Mus musculus activated spleen cDN... 34.2 18
gi|74139243|dbj|AK165986.1| Mus musculus lung RCB-0558 LLC cD... 34.2 18
gi|46358470|gb|AC148688.1| Macaca mulatta Major Histocompatib... 34.2 18
gi|110083219|emb|CT757481.6| Pig DNA sequence from clone CH24... 34.2 18
gi|62822550|gb|AC159040.2| Pan troglodytes BAC clone CH251-14... 34.2 18
gi|62702371|gb|AC156807.2| Pan troglodytes BAC clone CH251-35... 34.2 18
gi|47118306|dbj|BA000025.2| Homo sapiens genomic DNA, chromosome 34.2 18
gi|6997384|dbj|AP001048.1| Homo sapiens genomic DNA, chromoso... 34.2 18

Alignments

>gi|33285446|ref|NM_012567.2| Rattus norvegicus gap junction membrane channel protein alpha
1 (Gja1), mRNA
Length=3148

Score = 42.1 bits (21), Expect = 0.075
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||||||||||
Sbjct 1030 CTCGCCTATGTCTCCTCCTGG 1050

>gi|18250447|emb|AL139098.15| Human DNA sequence from clone RP1-276J11 on chromosome 6 Contains
the 5' end of the gene for a novel protein, a ribosomal
protein S15 (RPS15) pseudogene, a putative novel gene and the
GJA1 gene for gap junction protein, alpha 1, 43 kD (connexin
43) (CX43). Contains a CpG island, complete sequence
Length=131501

Score = 42.1 bits (21), Expect = 0.075
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||||||||||
Sbjct 113839 CTCGCCTATGTCTCCTCCTGG 113859

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 114089 GCTGGTCCACAATGGCTAGT 114070

>gi|20072865|gb|BC026329.1| Homo sapiens gap junction protein, alpha 1, 43kDa (connexin 43),
mRNA (cDNA clone MGC:26323 IMAGE:4794131), complete cds
Length=3000

Score = 42.1 bits (21), Expect = 0.075
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||||||||||
Sbjct 922 CTCGCCTATGTCTCCTCCTGG 942

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 1172 GCTGGTCCACAATGGCTAGT 1153

>gi|51859469|gb|BC081842.1| Rattus norvegicus gap junction membrane channel protein alpha
1, mRNA (cDNA clone MGC:93610 IMAGE:7105924), complete cds
Length=3156

Score = 42.1 bits (21), Expect = 0.075
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||||||||||
Sbjct 1026 CTCGCCTATGTCTCCTCCTGG 1046

>gi|55978|emb|X06656.1|RNCONN43 Rat heart mRNA for gap junction protein connexin43
Length=2768

Score = 42.1 bits (21), Expect = 0.075
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||||||||||
Sbjct 1035 CTCGCCTATGTCTCCTCCTGG 1055

>gi|4755136|ref|NM_000165.2| Homo sapiens gap junction protein, alpha 1, 43kDa (connexin 43)
(GJA1), mRNA
Length=3088

Score = 42.1 bits (21), Expect = 0.075
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||||||||||
Sbjct 1041 CTCGCCTATGTCTCCTCCTGG 1061

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 1291 GCTGGTCCACAATGGCTAGT 1272

>gi|109072851|ref|XM_001109617.1| PREDICTED: Macaca mulatta connexin 43, transcript variant 2 (GJA1),
mRNA
Length=3171

Score = 42.1 bits (21), Expect = 0.075
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||||||||||
Sbjct 1105 CTCGCCTATGTCTCCTCCTGG 1125

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 1355 GCTGGTCCACAATGGCTAGT 1336

>gi|109072849|ref|XM_001109566.1| PREDICTED: Macaca mulatta connexin 43, transcript variant 1 (GJA1),
mRNA
Length=3121

Score = 42.1 bits (21), Expect = 0.075
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||||||||||
Sbjct 1055 CTCGCCTATGTCTCCTCCTGG 1075

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 1305 GCTGGTCCACAATGGCTAGT 1286

>gi|39981118|gb|AY382588.1| Cercopithecus aethiops connexin 43 gene, partial cds
Length=1146

Score = 42.1 bits (21), Expect = 0.075
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||||||||||
Sbjct 834 CTCGCCTATGTCTCCTCCTGG 854

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 1084 GCTGGTCCACAATGGCTAGT 1065

>gi|67971111|dbj|AB169817.1| Macaca fascicularis brain cDNA, clone: QflA-10385, similar to
human gap junction protein, alpha 1, 43kDa (connexin 43)(GJA1),
mRNA, RefSeq: NM_000165.2
Length=3071

Score = 42.1 bits (21), Expect = 0.075
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||||||||||
Sbjct 1008 CTCGCCTATGTCTCCTCCTGG 1028

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 1258 GCTGGTCCACAATGGCTAGT 1239

>gi|49456280|emb|CR541660.1| Homo sapiens full open reading frame cDNA clone RZPDo834A1127D
for gene GJA1, gap junction protein, alpha 1, 43kDa (connexin
43); complete cds, without stopcodon
Length=1146

Score = 42.1 bits (21), Expect = 0.075
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||||||||||
Sbjct 834 CTCGCCTATGTCTCCTCCTGG 854

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 1084 GCTGGTCCACAATGGCTAGT 1065

>gi|32816616|gb|AY324140.1| Rattus norvegicus gap junction membrane channel protein alpha1
(Gja1) mRNA, complete cds
Length=3148

Score = 42.1 bits (21), Expect = 0.075
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||||||||||
Sbjct 1030 CTCGCCTATGTCTCCTCCTGG 1050

>gi|6563407|gb|AF151980.2|AF151980 Homo sapiens connexin 43 (CX43) gene, complete cds
Length=1149

Score = 42.1 bits (21), Expect = 0.075
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||||||||||
Sbjct 834 CTCGCCTATGTCTCCTCCTGG 854

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 1084 GCTGGTCCACAATGGCTAGT 1065

>gi|62898138|dbj|AK223289.1| Homo sapiens mRNA for connexin 43 variant, clone: SYN08454
Length=1828

Score = 42.1 bits (21), Expect = 0.075
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||||||||||
Sbjct 1037 CTCGCCTATGTCTCCTCCTGG 1057

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 1287 GCTGGTCCACAATGGCTAGT 1268

>gi|60654370|gb|AY892959.1| Synthetic construct Homo sapiens clone FLH140321.01L gap junction
protein alpha 1 (GJA1) mRNA, partial cds
Length=1149

Score = 42.1 bits (21), Expect = 0.075
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||||||||||
Sbjct 834 CTCGCCTATGTCTCCTCCTGG 854

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 1084 GCTGGTCCACAATGGCTAGT 1065

>gi|61363755|gb|AY890497.1| Synthetic construct Homo sapiens clone FLH140325.01X gap junction
protein alpha 1 (GJA1) mRNA, complete cds
Length=1149

Score = 42.1 bits (21), Expect = 0.075
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||||||||||
Sbjct 834 CTCGCCTATGTCTCCTCCTGG 854

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 1084 GCTGGTCCACAATGGCTAGT 1065

>gi|60827372|gb|AY893759.1| Synthetic construct Homo sapiens clone FLH131079.01L gap junction
protein alpha 1 (GJA1) mRNA, partial cds
Length=1149

Score = 42.1 bits (21), Expect = 0.075
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||||||||||
Sbjct 834 CTCGCCTATGTCTCCTCCTGG 854

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 1084 GCTGGTCCACAATGGCTAGT 1065

>gi|29916|emb|X52947.1|HSCGJP Human mRNA for cardiac gap junction protein
Length=3038

Score = 42.1 bits (21), Expect = 0.075
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||||||||||
Sbjct 991 CTCGCCTATGTCTCCTCCTGG 1011

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 1241 GCTGGTCCACAATGGCTAGT 1222

>gi|181208|gb|M65188.1|HUMCX43 Human connexin 43 (GJA1, Cx43) mRNA, complete cds
Length=1314

Score = 42.1 bits (21), Expect = 0.075
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||||||||||
Sbjct 999 CTCGCCTATGTCTCCTCCTGG 1019

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 1249 GCTGGTCCACAATGGCTAGT 1230

>gi|39981122|gb|AY382590.1| Oryctolagus cuniculus connexin 43 gene, partial cds
Length=1146

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 1084 GCTGGTCCACAATGGCTAGT 1065

>gi|38609727|gb|AY462223.1| Canis familiaris connexin 43 (Gja1) mRNA, complete cds
Length=1251

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 1084 GCTGGTCCACAATGGCTAGT 1065

>gi|21844555|gb|AC010228.8| Homo sapiens chromosome 5 clone CTC-298B17, complete sequence
Length=78680

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 18584 GCTGGTCCACAATGGCTAGT 18565

Score = 34.2 bits (17), Expect = 18
Identities = 20/21 (95%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||| ||||||
Sbjct 18334 CTCGCCTATGTCTCTTCCTGG 18354

>gi|34419626|ref|NG_003029.1| Homo sapiens gap junction protein, alpha 1, 43kDa (connexin 43)
pseudogene 1 (GJA1P1) on chromosome 5
Length=3272

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 1380 GCTGGTCCACAATGGCTAGT 1361

Score = 34.2 bits (17), Expect = 18
Identities = 20/21 (95%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||| ||||||
Sbjct 1130 CTCGCCTATGTCTCTTCCTGG 1150

>gi|6102634|gb|AC011421.2|AC011421 Homo sapiens chromosome 5 clone CTD-2303O5, complete sequence
Length=148142

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 105771 GCTGGTCCACAATGGCTAGT 105752

Score = 34.2 bits (17), Expect = 18
Identities = 20/21 (95%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||| ||||||
Sbjct 105521 CTCGCCTATGTCTCTTCCTGG 105541

>gi|50950160|ref|NM_001002951.1| Canis familiaris gap junction protein, alpha 1, 43kDa (connexin
43) (GJA1), mRNA
Length=1251

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 1084 GCTGGTCCACAATGGCTAGT 1065

>gi|181210|gb|M65189.1|HUMCX43PS Human connexin 43 processed pseudogene
Length=1397

Score = 40.1 bits (20), Expect = 0.29
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAGT 40
||||||||||||||||||||
Sbjct 1332 GCTGGTCCACAATGGCTAGT 1313

Score = 34.2 bits (17), Expect = 18
Identities = 20/21 (95%), Gaps = 0/21 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGG 21
|||||||||||||| ||||||
Sbjct 1082 CTCGCCTATGTCTCTTCCTGG 1102

>gi|28975324|gb|AY206456.1| Cricetulus griseus gap junction protein connexin 43 mRNA, complete
cds
Length=1477

Score = 38.2 bits (19), Expect = 1.2
Identities = 19/19 (100%), Gaps = 0/19 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAG 39
|||||||||||||||||||
Sbjct 1274 GCTGGTCCACAATGGCTAG 1256

Score = 36.2 bits (18), Expect = 4.6
Identities = 18/18 (100%), Gaps = 0/18 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCC 18
||||||||||||||||||
Sbjct 1024 CTCGCCTATGTCTCCTCC 1041

>gi|28975322|gb|AY206455.1| Mesocricetus auratus gap junction protein connexin 43 mRNA, complete
cds
Length=1488

Score = 38.2 bits (19), Expect = 1.2
Identities = 19/19 (100%), Gaps = 0/19 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAG 39
|||||||||||||||||||
Sbjct 1277 GCTGGTCCACAATGGCTAG 1259

Score = 36.2 bits (18), Expect = 4.6
Identities = 18/18 (100%), Gaps = 0/18 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCC 18
||||||||||||||||||
Sbjct 1027 CTCGCCTATGTCTCCTCC 1044

>gi|39981128|gb|AY382593.1| Sus scrofa connexin 43 gene, partial cds
Length=1146

Score = 38.2 bits (19), Expect = 1.2
Identities = 19/19 (100%), Gaps = 0/19 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAG 39
|||||||||||||||||||
Sbjct 1084 GCTGGTCCACAATGGCTAG 1066

>gi|39981126|gb|AY382592.1| Spermophilus citellus connexin 43 gene, partial cds
Length=1146

Score = 38.2 bits (19), Expect = 1.2
Identities = 19/19 (100%), Gaps = 0/19 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAG 39
|||||||||||||||||||
Sbjct 1084 GCTGGTCCACAATGGCTAG 1066

Score = 36.2 bits (18), Expect = 4.6
Identities = 18/18 (100%), Gaps = 0/18 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCC 18
||||||||||||||||||
Sbjct 834 CTCGCCTATGTCTCCTCC 851

>gi|39981124|gb|AY382591.1| Phodopus sungorus connexin 43 gene, partial cds
Length=1146

Score = 38.2 bits (19), Expect = 1.2
Identities = 19/19 (100%), Gaps = 0/19 (0%)
Strand=Plus/Minus

Query 21 GCTGGTCCACAATGGCTAG 39
|||||||||||||||||||
Sbjct 1084 GCTGGTCCACAATGGCTAG 1066

Score = 36.2 bits (18), Expect = 4.6
Identities = 18/18 (100%), Gaps = 0/18 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCC 18
||||||||||||||||||
Sbjct 834 CTCGCCTATGTCTCCTCC 851

>gi|42544094|gb|AC131105.4| Mus musculus BAC clone RP23-280N9 from chromosome 19, complete
sequence
Length=225527

Score = 38.2 bits (19), Expect = 1.2
Identities = 25/27 (92%), Gaps = 0/27 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCCTGGCTGGTC 27
||||||| |||||||||||||| ||||
Sbjct 47637 CTCGCCTTTGTCTCCTCCTGGCAGGTC 47663

>gi|39752885|gb|AC132957.3| Mus musculus BAC clone RP23-149E9 from chromosome 19, complete
sequence
Length=246177

Score = 38.2 bits (19), Expect = 1.2
Identities = 25/27 (92%), Gaps = 0/27 (0%)
Strand=Plus/Minus

Query 1 CTCGCCTATGTCTCCTCCTGGCTGGTC 27
||||||| |||||||||||||| ||||
Sbjct 7969 CTCGCCTTTGTCTCCTCCTGGCAGGTC 7943

>gi|55294919|emb|BX547998.9| Zebrafish DNA sequence from clone DKEYP-118B1, complete sequence
Length=47625

Score = 36.2 bits (18), Expect = 4.6
Identities = 24/26 (92%), Gaps = 0/26 (0%)
Strand=Plus/Minus

Query 8 ATGTCTCCTCCTGGCTGGTCCACAAT 33
|||||| |||| ||||||||||||||
Sbjct 38052 ATGTCTACTCCAGGCTGGTCCACAAT 38027

>gi|22213375|gb|AC104036.8| Homo sapiens chromosome 8, clone RP11-665N23, complete sequence
Length=183763

Score = 36.2 bits (18), Expect = 4.6
Identities = 18/18 (100%), Gaps = 0/18 (0%)
Strand=Plus/Minus

Query 7 TATGTCTCCTCCTGGCTG 24
||||||||||||||||||
Sbjct 48280 TATGTCTCCTCCTGGCTG 48263

>gi|71725580|gb|AC155807.3| Mus musculus BAC clone RP24-303N21 from chromosome 8, complete
sequence
Length=167996

Score = 36.2 bits (18), Expect = 4.6
Identities = 18/18 (100%), Gaps = 0/18 (0%)
Strand=Plus/Minus

Query 6 CTATGTCTCCTCCTGGCT 23
||||||||||||||||||
Sbjct 111353 CTATGTCTCCTCCTGGCT 111336

>gi|21070323|gb|AF508037.1| Mesocricetus auratus connexin 47 mRNA, partial cds
Length=482

Score = 36.2 bits (18), Expect = 4.6
Identities = 18/18 (100%), Gaps = 0/18 (0%)
Strand=Plus/Plus

Query 1 CTCGCCTATGTCTCCTCC 18
||||||||||||||||||
Sbjct 368 CTCGCCTATGTCTCCTCC 385

>gi|26087706|dbj|AK040228.1| Mus musculus 0 day neonate thymus cDNA, RIKEN full-length enriched
library, clone:A430079I04 product:unclassifiable, full
insert sequence
Length=2783

Score = 36.2 bits (18), Expect = 4.6
Identities = 18/18 (100%), Gaps = 0/18 (0%)
Strand=Plus/Plus

Query 10 GTCTCCTCCTGGCTGGTC 27
||||||||||||||||||
Sbjct 496 GTCTCCTCCTGGCTGGTC 513

>gi|19849385|gb|AC015476.10| Homo sapiens, clone RP11-16G11, complete sequence
Length=154768

Score = 36.2 bits (18), Expect = 4.6
Identities = 18/18 (100%), Gaps = 0/18 (0%)
Strand=Plus/Plus

Query 7 TATGTCTCCTCCTGGCTG 24
||||||||||||||||||
Sbjct 28091 TATGTCTCCTCCTGGCTG 28108

>gi|68378929|ref|XM_683374.1| PREDICTED: Danio rerio similar to ataxin 7-like 3 (LOC559983),
mRNA
Length=797

Score = 36.2 bits (18), Expect = 4.6
Identities = 24/26 (92%), Gaps = 0/26 (0%)
Strand=Plus/Minus

Query 8 ATGTCTCCTCCTGGCTGGTCCACAAT 33
|||||| |||| ||||||||||||||
Sbjct 499 ATGTCTACTCCAGGCTGGTCCACAAT 474

>gi|68342226|gb|AC152718.6| Mus musculus BAC clone RP24-432B3 from chromosome 9, complete
sequence
Length=174264

Score = 36.2 bits (18), Expect = 4.6
Identities = 18/18 (100%), Gaps = 0/18 (0%)
Strand=Plus/Plus

Query 10 GTCTCCTCCTGGCTGGTC 27
||||||||||||||||||
Sbjct 22812 GTCTCCTCCTGGCTGGTC 22829

>gi|22779223|dbj|AP005358.3| Homo sapiens genomic DNA, chromosome 8q23, clone: KB1356F10,
complete sequence
Length=159115

Score = 36.2 bits (18), Expect = 4.6
Identities = 18/18 (100%), Gaps = 0/18 (0%)
Strand=Plus/Minus

Query 7 TATGTCTCCTCCTGGCTG 24
||||||||||||||||||
Sbjct 15800 TATGTCTCCTCCTGGCTG 15783

>gi|8072467|gb|AC009567.8|AC009567 Homo sapiens chromosome 4, clone RP11-21G20, complete sequence
Length=164823

Score = 36.2 bits (18), Expect = 4.6
Identities = 18/18 (100%), Gaps = 0/18 (0%)
Strand=Plus/Minus

Query 5 CCTATGTCTCCTCCTGGC 22
||||||||||||||||||
Sbjct 30021 CCTATGTCTCCTCCTGGC 30004

>gi|25141111|gb|AC084125.9| Homo sapiens chromosome 8, clone CTD-2517M22, complete sequence
Length=197294

Score = 36.2 bits (18), Expect = 4.6
Identities = 18/18 (100%), Gaps = 0/18 (0%)
Strand=Plus/Minus

Query 14 CCTCCTGGCTGGTCCACA 31
||||||||||||||||||
Sbjct 37203 CCTCCTGGCTGGTCCACA 37186

>gi|61403389|gb|BC091904.1| Danio rerio cDNA clone IMAGE:7149102
Length=1934

Score = 36.2 bits (18), Expect = 4.6
Identities = 24/26 (92%), Gaps = 0/26 (0%)
Strand=Plus/Minus

Query 8 ATGTCTCCTCCTGGCTGGTCCACAAT 33
|||||| |||| ||||||||||||||
Sbjct 483 ATGTCTACTCCAGGCTGGTCCACAAT 458

>gi|21727418|emb|AL672195.9| Mouse DNA sequence from clone RP23-468E16 on chromosome 9, complete
sequence
Length=182503

Score = 36.2 bits (18), Expect = 4.6
Identities = 18/18 (100%), Gaps = 0/18 (0%)
Strand=Plus/Minus

Query 10 GTCTCCTCCTGGCTGGTC 27
||||||||||||||||||
Sbjct 89653 GTCTCCTCCTGGCTGGTC 89636

>gi|25896305|emb|AL928738.8| Mouse DNA sequence from clone RP23-76M15 on chromosome 4, complete
sequence
Length=188568

Score = 36.2 bits (18), Expect = 4.6
Identities = 18/18 (100%), Gaps = 0/18 (0%)
Strand=Plus/Minus

Query 17 CCTGGCTGGTCCACAATG 34
||||||||||||||||||
Sbjct 42998 CCTGGCTGGTCCACAATG 42981

>gi|76253341|emb|CR974460.5| Pig DNA sequence from clone PigE-150I6 on chromosome 7, complete
sequence
Length=122162

Score = 34.2 bits (17), Expect = 18
Identities = 17/17 (100%), Gaps = 0/17 (0%)
Strand=Plus/Minus

Query 11 TCTCCTCCTGGCTGGTC 27
|||||||||||||||||
Sbjct 79138 TCTCCTCCTGGCTGGTC 79122

>gi|21687178|ref|NM_010288.2| Mus musculus gap junction membrane channel protein alpha 1 (Gja1),
mRNA
Length=3071

Score = 34.2 bits (17), Expect = 18
Identities = 17/17 (100%), Gaps = 0/17 (0%)
Strand=Plus/Plus

Query 5 CCTATGTCTCCTCCTGG 21
|||||||||||||||||
Sbjct 1023 CCTATGTCTCCTCCTGG 1039

>gi|71274021|emb|CR942185.8| Human DNA sequence from clone DAMC-81L12 on chromosome 6, complete
sequence
Length=55481

Score = 34.2 bits (17), Expect = 18
Identities = 17/17 (100%), Gaps = 0/17 (0%)
Strand=Plus/Minus

Query 9 TGTCTCCTCCTGGCTGG 25
|||||||||||||||||
Sbjct 45145 TGTCTCCTCCTGGCTGG 45129



Database: All GenBank+EMBL+DDBJ+PDB sequences (but no EST, STS, GSS,environmental
samples or phase 0, 1 or 2 HTGS sequences)
Posted date: Aug 10, 2006 8:25 PM
Number of letters in database: 756,130,373
Number of sequences in database: 4,309,818
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Sequences: 4309818
Number of Hits to DB: 18075970
Number of extensions: 7664803
Number of successful extensions: 10095
Number of sequences better than 1000: 226
Number of HSP's better than 1000 without gapping: 0
Number of HSP's gapped: 10095
Number of HSP's successfully gapped: 233
Length of query: 40
Length of database: 17935999557
Length adjustment: 20
Effective length of query: 20
Effective length of database: 17849803197
Effective search space: 356996063940
Effective search space used: 356996063940
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
X3: 25 (49.6 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)